核(he)酸表(biao)觀遺傳(chuan)是(shi)在(zai)堿(jian)基(ji)序列(lie)不(bu)變(bian)的情(qing)況下(xia)引(yin)起基(ji)因功能改變(bian)的遺傳(chuan)機(ji)制(zhi).其(qi)中(zhong),5-羥(qiang)甲基(ji)胞嘧(mi)啶(5hmC)被(bei)稱為哺(bu)乳(ru)動(dong)物(wu)DNA的"第六(liu)堿(jian)基(ji)",受(shou)到(dao)了廣(guang)泛(fan)關(guan)註(zhu).作為(wei)另壹種(zhong)羥(qiang)甲基(ji)嘧(mi)啶,5-羥(qiang)甲基(ji)尿(niao)嘧(mi)啶(5hmU)可(ke)通過5hmC脫(tuo)氨或胸腺(xian)嘧(mi)啶氧(yang)化(hua)等途(tu)徑生(sheng)成(cheng),在(zai)細(xi)胞內(nei)發(fa)揮(hui)重要(yao)作用.

        然而,5hmU的化(hua)學(xue)結構與(yu)5hmC高(gao)度(du)相似,且其細胞豐度不到後者的1%.因此,兩(liang)者的特(te)異(yi)識(shi)別、區分極(ji)其(qi)困難(nan),更(geng)無(wu)法(fa)在(zai)單(dan)細胞水(shui)平分析並探(tan)究(jiu)其細胞功能單(dan)細胞5hmU/5hmC單(dan)分子(zi)可(ke)視(shi)化(hua)分析示(shi)意(yi)圖針(zhen)對(dui)上述(shu)問題,西(xi)安交(jiao)通大學(xue)生(sheng)命學(xue)院(yuan)生(sheng)命分析化學(xue)與(yu)儀(yi)器研(yan)究(jiu)所(suo)趙(zhao)永(yong)席教(jiao)授團(tuan)隊發(fa)展(zhan)了可(ke)特異(yi)區分5hmU與(yu)5hmC的(de)化學(xue)酶(chemoenzymatic)標(biao)記(ji)法(fa),並(bing)構(gou)建單(dan)細胞凝(ning)膠(jiao)液滴(di)微(wei)流控(kong)DNA擴(kuo)增(zeng)檢測系(xi)統(tong),實(shi)現(xian)單(dan)細胞5hmU/5hmC的(de)單(dan)分子(zi)可(ke)視(shi)化(hua)分析,揭示(shi)細胞類(lei)型特(te)異(yi)的表(biao)觀修(xiu)飾(shi)位(wei)點數(shu)目、分布(bu)規(gui)律(lv)等分子(zi)特(te)征,並利(li)用深度學(xue)習算法(fa),首次(ci)證(zheng)實(shi)5hmU與(yu)5hmC可(ke)作為(wei)標誌物(wu),區分正(zheng)常(chang)、致(zhi)癌、高(gao)度(du)轉(zhuan)移的乳腺細(xi)胞系(xi)相(xiang)關(guan)成果(guo)以《微(wei)流凝(ning)膠(jiao)編碼(ma)的(de)單(dan)細胞5hmU/5hmC可(ke)視(shi)化(hua)分析》 (Differentiated Visualization of Single-Cell 5Hydroxymethylpyrimidines with Microfluidic Hydrogel Encoding)為題發(fa)表(biao)在(zai)國(guo)際(ji)化(hua)學(xue)領域權威(wei)期(qi)刊(kan)《美(mei)國(guo)化(hua)學(xue)會(hui)誌(zhi)》(Journal of the American Chemical Society)上.

         該論(lun)文第壹(yi)作者為西(xi)安交(jiao)大生(sheng)命學(xue)院(yuan)陳鋒副教(jiao)授與(yu)博(bo)士(shi)生(sheng)薛(xue)靜(jing),通訊作者是(shi)西(xi)安交(jiao)大生(sheng)命學(xue)院(yuan)趙永(yong)席教(jiao)授,第壹(yi)單(dan)位(wei)為西(xi)安交(jiao)大生(sheng)物(wu)醫學(xue)信(xin)息(xi)工(gong)程(cheng)教(jiao)育(yu)部重點實(shi)驗(yan)室(shi).上海交通大學(xue)樊春(chun)海院(yuan)士(shi)對(dui)該工作提供了重要(yao)的指(zhi)導(dao).該項研(yan)究(jiu)得到(dao)國(guo)家(jia)自(zi)然科學(xue)基(ji)金、西(xi)安交(jiao)大青(qing)年拔尖人(ren)才計劃(hua)等多個項目的(de)共同(tong)資助.趙永(yong)席教(jiao)授課(ke)題組研(yan)究(jiu)方(fang)向(xiang)包括高(gao)通量單(dan)分子(zi)與(yu)單(dan)細胞分析、核(he)酸化(hua)學(xue)與(yu)生(sheng)物(wu)學(xue)、功能納米材料與(yu)分子(zi)探(tan)針等.近(jin)3年在(zai)《美(mei)國(guo)化(hua)學(xue)會(hui)誌(zhi)》(Journal of the American Chemical Society)、《德國(guo)應用化學(xue)》(Angewandte Chemie International Edition)、《核(he)酸研(yan)究(jiu)》(Nucleic Acids Research)等國(guo)際(ji)權威(wei)刊(kan)物(wu)上發(fa)表多篇(pian)論(lun)文.
專(zhuan)業制(zhi)作微(wei)流控(kong)芯(xin)片(pian)設(she)備我(wo)們(men)推(tui)薦奮進(jin)號(hao)HZZ-V3000(i.LASER3000/4000)詳(xiang)細了解(jie)請聯(lian)系(xi)我(wo)們.

 

 

 

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